Mulitple proteins embedded in small positively charged liposomes were delivered into the membranes of negatively charged giant unilamellar vesicles by charged mediated fusion. The process occurs within a few minutes and was verified by functional analysis of the proteins and fluorescence microscopy.

This work was carried out in the group of Prof. Christoph von Ballmoos.

References:

• O. Biner, T. Schick, Y. Müller, C. von Ballmoos;
  "Delivery of membrane proteins into small and giant unilamellar vesicles by charge-mediated fusion"
  FEBS Letters, 2016, 590(14), 2047; doi:10.1002/1873-3468.12302   &
  FEBS Letters, 2016, 590(14), 2051-2062; doi:10.1002/1873-3468.12233.