Molecule of the Month September 2009 |
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de-novo Identification of a Complex Disulfide Bridge Pattern | ||
The cystine knot structure of homodimeric recombinant human growth and differentiation factor 5 (rGDF5) was identified de novo applying a combination of proteolytic degradation and subsequent analyses of the disulfide-linked peptides by electrospray- and MALDI-TOF mass spectrometry, amino acid analysis, and Edman degradation.
This work was carried out in the groups of PD Dr. Stefan Schürch and Prof. Johann Schaller. References:
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