Molecule of the Month
June 2022
The Effects of Cycloheximide in Ribosome Profiling Experiments

Ribosome profiling is the gold-standard method to measure mRNA translation dynamics in living cells. However, it was unclear whether the widely used translation inhibitor cycloheximide (CHX) biases such analyses.
We showed that the effects of cycloheximide have been strongly overestimated and that its careful use is fully justified.

This work was carried out in the group of Prof. Dr. Sebastian Leidel.


  • P. Sharma, J. Wu, B. S. Nilges, S. A. Leidel;
    "Humans and other commonly used model organisms are resistant to cycloheximide mediated biases in ribosome profiling experiments"
    Nature Communications, 2021, 12(1), 5094/1-13; doi:10.1038/s41467-021-25411-y.