tRNAs are well-known for their role in protein biosynthesis. More recently, tRNA-derived fragments were discovered as novel non-coding RNAs class in all domains of life. They can control gene expression at various levels (e.g. as ribosome-associated RNA) and their mis-regulation is connected to human diseases.
Norbert Polacek, together with a colleague of the Harvard Medical School Boston, has compiled a Methods in Enzymology book issue dedicated to tRNA-derived RNA research.

Two chapters in this book were contributed by Alessia Rosina, Carmela Esposito & colleagues from the group of Prof. Dr. Norbert Polacek.

References:

• C. Esposito, A. Buzoianu, M. Cristodero, N. Polacek;
  "Ribozyme-mediated expression of tRNA-derived small RNAs in bacteria"
  Methods in Enzymology, 2024, 711, in press; doi:10.1016/bs.mie.2024.11.003.
• A. Rosina, N. Polacek, R. Rauscher;
  "Ribosome-associated tDRs in yeast"
  Methods in Enzymology, 2024, 711, in press; doi:10.1016/bs.mie.2024.11.006.