Cell-free translation:

• A technique to produce proteins outside of living cells using cell extracts
• Facilitates protein synthesis research, synthetic biology, drug discovery, production of toxic proteins

Our innovation:

• Developed a scalable, efficient method using dual centrifugation from diverse human cell lines
• Enables cell-type-specific studies of translation and post-translational modifications

Developed in the groups of Prof. Dr. Oliver Mühlemann and PD Dr. Evangelos Karousis.

References:

• J. Ziegelmüller, N. Kouvelas, P. Thambythurai, A. M. Hofer, O. Mühlemann, E. D. Karousis;
  "Efficient cell-free translation from diverse human cell types"
  bioRxiv, 2024.12.16.628677, preprint; doi:10.1101/2024.12.16.628677.